Effects of RNAi-mediated hnRNPA2/B1 knock-down on porcine pre-implantation embryo development. (A) hnRNPA2/B1 ICC image between control and knock-down 2-cell embryos. After 24H of microinjection of dsRNA, embryos were carried out immunostaining. Scale bars = 50 μM. (B) hnRNPA2/B1 protein intensity and (C) mRNA levels in the hnRNPA2/B1 dsRNA injected embryos were examined. (D, E) Developmental rates of porcine pre-implantation embryos injected with hnRNPA2/B1 dsRNA and control. (D) Representative images of HNRNPA2/B1 knock-down and control embryos at blastocyst stages after 144H microinjection. Scale bars = 500 μM. (E) Blastocyst rates was calculated of expanded and clear cavity formation blastocyst. Three experimental replicates were examined using 100-150 embryos per group. ***p < 0.01.