Post-thaw viability and acrosome integrity of Siberian tiger epidydimal spermatozoa cryopreserved with modified Norwegian extender. (A) Live spermatozoa stained with SYBR® 14. Magnification at 100x. (B) Dead spermatozoa stained with propidium iodide. Magnification at 100x. Arrow indicates artifacts. (C) Intact acrosome of spermatozoa stained with Pisum Sativum Agglutinin labelled with FITC. (D) A graph shows the results of viability and acrosome integrity. Values are expressed as mean ± SE.
pISSN: 2671-4639
