Morphological classification of bovine intestinal organoids. (A) Bovine intestinal organoids were classified into various morphologies, such as spheroidal (round shaped), stomatocyte, budding (spheroids with extension) and mature villi and crypt-like structures, at each passage. Spherical: 200 µm, Stomatocyte: 50 µm, Budded/Elongated: 100 µm, Branched: 100 µm. (B) The number of intestinal organoids at spontaneous differentiation according to each morphology was counted at 3-day intervals before passaging. Significant differences between groups were analyzed by two-way ANOVA of variance. A p value less than or equal to 0.05 indicated statistical significance (*p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ 0.001).