Overall sublethal effects of norflurazon on zebrafish in the developmental stage. (A) Percentage of surviving zebrafish embryos at 96 h post-fertilization (hpf) following norflurazon treatment. Error bars represent standard deviations from triplicate data. (B) Percentage of hatched zebrafish embryos at 24, 48, 72, and 96 hpf. Error bars represent standard deviations from triplicate data. (C) Overall morphology at 96 hpf of zebrafish exposed to norflurazon. Scale bar represents 500 μm. (D) Representative image showing morphological abnormalities induced by norflurazon treatment. Green arrow: body length, quantified in (E). Blue arrow: yolk sac area, quantified in (F). Orange arrow: pericardial area with cardiac edema, quantified in (G). Bar graphs present the (E) relative body length, (F) relative yolk sac area, and (G) relative pericardial area of the treatment group compared with the vehicle group. Asterisks denote the significance level: *p < 0.05, **p < 0.01, and ***p < 0.001.