Analysis of apoptotic cell death following norflurazon treatment. (A) Captured images of zebrafish stained with acridine orange (A.O) at 96 h post-fertilization (hpf). Scale bar represents 300 μm. (B) Green fluorescence intensity representing the number of stained apoptotic cells. The fluorescence intensity was quantified with an image of norflurazon-treated zebrafish incubated with acridine orange using ImageJ software. Asterisks denote the significance level: **p < 0.01 and ***p < 0.001.