Effects of Mito-TEMPO on the blastocyst developmental rate and F-actin structure in bovine embryos. (A) Representative images of blastocysts according to the 0.1 μM Mito-TEMPO treatment in bovine embryos. Scale bar = 500 μm. (B) The percentage of expanded blastocysts derived from Mito-TEMPO treated bovine embryos. Scale bar = 100 μm. Data are expressed as mean ± SD and were analyzed using a t-test. Differences were considered significant at p < 0.05. (C) Accumulation of adhesive junction F-actin and aggregation F-actin in Mito-TEMPO treated bovine embryos. (D) Fluorescence expressions were presented with F-actin (green) and nuclei (blue) by immunofluorescent (IF) and DAPI staining in bovine blastocyst derived from Mito-TEMPO treated embryos. Scale bar = 50 μm. Data are presented in the bar graph as the mean ± SD from three independent experiments. Differences were considered significant at *p < 0.05 and **p < 0.01 in comparison to the non-treated group.