Changes of viability and F-actin aggregation in vitrified-warmed blastocysts from Mito-TEMPO treated bovine embryos. (A) Representative images of the survival morphologies in vitrified-warmed blastocysts from Mito-TEMPO treated bovine embryos. Scale bar = 500 μm. (B) The percentage of survival in vitrified-warmed blastocysts derived from Mito-TEMPO treated bovine embryos. Data are expressed as mean ± SD and were analyzed using a t-test. Differences were considered significant at p < 0.05. (C) Fluorescence expressions were presented with F-actin (green) and nuclei (blue) by IF and DAPI staining in Mito-TEMPO treated bovine vitrified-warmed blastocysts. Scale bar = 50 μm. (D) F-actin aggregation and fluorescence intensity in Mito-TEMPO treated bovine vitrified-warmed blastocysts. Data are presented in the bar graph as the mean ± SD from three independent experiments. Differences were considered significant at *p < 0.05 and **p < 0.01 as compared to the non-treated group.
pISSN: 2671-4639
