MYO7A targeting strategy. To disrupt MYO7A in rhesus macaque embryos, two sgRNAs were designed that target MYO7A exon 3. The underlined DNA sequence denotes the sgRNA sequence, and the letters highlighted in red represent the protospacer adjacent motif (PAM) sequence. Black arrows represent the PCR primer set #1 used for amplification of the target region (expected PCR product size = 350 bp). Gray arrows represent flanking primers of set #2, which are predicted to yield a PCR product of 800 bp.
pISSN: 2671-4639
