Journal of Animal Reproduction and Biotechnology

Table. 1.

Table 1

Sperm motility and kinematics following incubation time

	Incubation time (min)

	60	90	120
MOT (%)	78.8 ± 1.42^a	72.28 ± 2.23^a,b	66.91 ± 1.47^b
RPD (%)	67.37 ± 1.72^a	60.92 ± 2.6^a,b	56.68 ± 1.55^b
MDM (%)	8.3 ± 0.38	8.07 ± 0.42	6.79 ± 0.33
SLW (%)	3.12 ± 0.26	3.12 ± 0.3	3.38 ± 0.33
PRG (%)	75.68 ± 1.41^a	68.99 ± 2.4^a,b	63.46 ± 1.28^b
VCL (μm/s)	83.16 ± 2.72^a	76.28 ± 5.49^a,b	67.17 ± 2.06^b
VSL (μm/s)	41.3 ± 1.75	37.44 ± 1.65	35.69 ± 1.46
VAP (μm/s)	59.51 ± 2.01^a	53.3 ± 3.25^a,b	48.52 ± 1.97^b
LIN (%)	41.38 ± 1.28	37.62 ± 0.64	37.04 ± 1.23
STR (%)	69.41 ± 1.05	70.7 ± 1.59	73.53 ± 0.65
BCF (Hz)	5.69 ± 0.3	4.99 ± 0.26	4.79 ± 0.14
MAD (degree)	58.85 ± 2.89	52.89 ± 2.4	52.27 ± 2.62
WOB (%)	71.74 ± 1.01	70.42 ± 1.3	72.37 ± 1.02
DNC (μm²/s)	279.75 ± 20.9	243.65 ± 40.56	174.58 ± 10.59
DNM (μm)	8.02 ± 0.44	8.1 ± 0.78	6.9 ± 0.25
ALH (μm)	3.28 ± 0.15	3 ± 0.28	2.53 ± 0.1

Sperm motility and kinematics are presented as mean ± SEM, n = 5. Different superscripts (^a,b) within the same row indicate significant differences (p < 0.05) in the one-way ANOVA. MOT, Total sperm motility (%); RPD, Rapid sperm motility (%); MED, Medium sperm motility (%); SLW, Slow sperm motility (%); PRG, Progressive sperm motility (%); VCL, Curvilinear velocity (μm/s); VSL, Straight-line velocity (μm/s); VAP, Average path velocity (μm/s); LIN, linearity [%, (VSL/VCL) × 100]; STR, Straightness [%, (VSL/VAP) × 100]; BCF, Beat-cross frequency (Hz); MAD, Mean angular displacement (degree); WOB, Wobble [%, (VAP/VCL) × 100]; DNC, Dance [μm²/sec, (VCL × ALH)]; DNM, Mean dance [μm, (ALH/LIN)]; ALH, Mean amplitude of head lateral displacement (μm).

Journal of Animal Reproduction and Biotechnology 2022;37:285-91 https://doi.org/10.12750/JARB.37.4.285