Sperm capacitation status following incubation time. (A) Patterns of alive and acrosome reacted (AR pattern). (B) Patterns of alive and capacitated (B pattern). (C) Patterns of alive and non-capacitated (F pattern). Values with different superscripts (a,b) indicate significant differences between the control and treatment groups (p < 0.05). Data were evaluated by one-way ANOVA and represent means ± SEM, n = 9.