pISSN: 2671-4639
eISSN: 2671-4663
pISSN: 2671-4639
eISSN: 2671-4663
Summary of primer sequences for RT-PCR, real-time RT-PCR, and in situ hybridization and expected product sizes
| Primer | Sequence of forward (F) and reverse (R) primers (5’ → 3’) | Annealing temperature (℃) | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
| Real-time and RT-PCR | ||||
| F: CCTCTGGAGGACACTTCTGC | 60 | 246 | XM_005662842.3 | |
| R: GACCACCTGTGCACACACTC | ||||
| F: TGTGACTACTCCGCAGATGG | 60 | 203 | XM_021091372.1 | |
| R: CCAGAGCAGTGATTGTCAGC | ||||
| F: AAG CCA AGC ACT ATC ACA AGG AAT ACA | 60 | 172 | NM_001113217 | |
| R: TGC AAC ACC TTT CTG ACC TTT GG | ||||
| F: GCATTGTTGGCGGTTTCG | 60 | 81 | NM_001105309.1 | |
| R: AGACGCTGTGAAGCCAATCA | ||||
| F: AACAGTTCAGTAGTTATGAGCCAGA | 60 | 262 | DQ845178.1 | |
| R: AGATGTTCTCAAACGCTTCG | ||||
| In situ hybridization | ||||
| F: CCTCTGGAGGACACTTCTGC | 60 | 295 | XM_005662842.3 | |
| R: AGGACTGGCCGTTGTTGTAG | ||||
| F: TTAAAAGGGATCCGGGAAAG | 60 | 209 | XM_021091372.1 | |
| R: TCAGGTGCCGTGTCTAACAG |