Expression of CCN4 and CCN6 in conceptuses from Days 12 and 15 of pregnancy and chorioallantoic tissues during later pregnancy. (A) RT-PCR of CCN4 and CCN6 mRNA from pregnancy Days 12 and 15 conceptuses using total RNA. RPL7 was used as a loading control. RTase +/-, with (+) or without (-) reverse transcriptase; M, molecular marker; D12P Endo, endometrium on Day 12 of pregnancy; D15P Endo, endometrium on Day 15 of pregnancy; D12 Con, Day 12 conceptus; D15 Con, Day 15 conceptus. (B) Real-time RT-PCR analysis of the expression of CCN4 and CCN6 mRNA in chorioallantoic tissues on Days 30, 60, 90, and 114 of pregnancy. Data are reported as expressions relative to that detected on Day 30 of pregnancy after normalization of the transcript amount to the endogenous RPL7, UBB, and TBP control, and are presented as mean with standard error. RT-PCR, reverse transcription-polymerase chain reaction; RPL7, ribosomal protein L7; UBB, ubiquitin B; TBP, TATA binding protein; ns, not significant.