Effects of E2 (A), P4 (B), and IFNG (C) on the expression of CCN4 and CCN6 mRNAs in endometrial explant cultures. Endometrial explants from prepubertal gilts were cultured with 0, 5, 50, 500 pg/mL E2 (estradiol-17β) or 0, 0.3, 3, 30 ng/mL P4 (progesterone) and endometrial explants from gilts on Day 12 of the estrous cycle were cultured with 0, 1, 10, 100 ng/mL IFNG. The abundance of mRNA expression determined by real-time RT-PCR analyses was relative to that for CCN4 and CCN6 mRNAs in the control group (0 ng/mL) of endometrial explants after normalization of transcript amounts to RPL7, UBB, and TBP mRNA. Data are presented as mean with standard error. These treatments were performed in triplicate using tissues obtained from each of the three gilts. RT-PCR, reverse transcription-polymerase chain reaction; RPL7, ribosomal protein L7; UBB, ubiquitin B; TBP, TATA binding protein; ns, not significant.