Effects of IL10 on the expression of CCN6 mRNAs in pUE cell cultures. pUE cells were cultured with 0, 1, 10, or 100 ng/mL IL10. The abundance of mRNA expression determined by real-time RT-PCR analyses was relative to that for CCN4 and CCN6 mRNAs in the control group (0 ng/mL) of pUE cells after normalization of transcript amounts to RPL7,UBB, and TBP mRNA. Data are presented as mean with standard error. These treatments were performed in triplicate using tissues obtained from each of the three gilts. pUE, porcine uterine epithelial; RT-PCR, reverse transcription-polymerase chain reaction; RPL7, ribosomal protein L7; UBB, ubiquitin B; TBP, TATA binding protein; ns, not significant.