Whole eye transplantation of human iPSC-derived eye from interspecies blastocyst complementation. (A) Obtain human somatic cells. (B) Obtain iPSC by reprogramming somatic cells using Yamanaka factors. (C) Injection of human iPSC into eye-disabled (Pax6 deactivated) pig blastocyst resulting in chimeric pig blastocyst. (D) Chimeric pig blastocyst is transferred to the oviduct of the recipient female pig. (E) Select chimeric offspring with human iPSC-derived eyes. (F) Enucleate iPSC-derived human eye from the chimeric offspring and transplant the whole eye into a human followed by nerve reconnection and revascularization.
pISSN: 2671-4639
