Table 1

Primer sequences and PCR cycling conditions

Genes	GenBank number	Primer sequence		Size (bp)	Temp (℃)
		Sense	Anti-sense
CD44	NM_174013.3	5’-CGGAACATAGGGTTTGAGA-3’	5’-GGTTGATGTCTTCTGGGTTA-3’	301	60
CD73	NM_174129.3	5’-CAATGGCACGATTACCTG-3’	5’-GACCTTCAACTGCTGGATA-3’	428	60
CD34	NM_174009.1	5’-CCTCATCAGCTTTGCGACTT-3’	5’-CCAGGAGCAAGGAGCACA-3’	314	60
GAPDH	NM_001034034.2	5’-AAAGTGGACATCGTCGCCAT-3’	5’-CCAGCATCACCCCACTTGAT-3’	194	60

Journal of Animal Reproduction and Biotechnology 2023;38:10-6 https://doi.org/10.12750/JARB.38.1.10