Number of intermuscular adipose tissue-derived primary cells isolated using different intermuscular adipose tissue dissociation protocols. Each primary cell was derived from intermuscular adipose tissues according to the experimental design. The total number of viable cells not stained with trypan blue dye within intermuscular adipose tissue-derived primary cells was determined by hemocytometry. In all experimental replicates, the total number of live primary cells obtained using Method 2 was greater than Method 1. Dotted lines indicate the mean of three independent experiments. Rep, replicate.