Number and size of CFU-Fs formed from intermuscular adipose tissue-derived primary cells isolated using different dissociation protocols and the total number of cells retrieved from the formed CFU-Fs. Primary cells retrieved from intermuscular adipose tissues using Method 1 and Method 2 were seeded onto culture plates and cultured for 8 days. Subsequently, the number (B) and size (C) of CFU-Fs consisting of more than 50 cells with typical fibroblast morphology (A) and total number (D) of cells retrieved from the formed CFU-Fs were counted. The methodological difference in the isolation of primary cells from intermuscular adipose tissue did not show any significant difference in the number of CFU-Fs formed from primary cells. In all experimental replicates, primary cells obtained using Method 2 resulted in larger CFU-Fs and a greater total number of cells retrieved from the formed CFU-Fs than Method 1. All data shown in (B) represent mean ± standard error of the mean (SEM) of four independent experiments. Dotted lines in (C) and (D) indicate the mean of three or four independent experiments. Scale bar in (A) is 200 μm. Rep, replicate.