Transcriptional expression of ADMSC-related genes in intermuscular ADMSCs cultured until passage 5 following isolation from intermuscular adipose tissues using different dissociation protocols. Intermuscular ADMSCs were established by forming CFU-Fs from intermuscular adipose tissue-derived primary cells isolated using Method 1 or Method 2. Quantitative monitoring of ADMSC-related genes CD44 and CD73 and the hematopoietic stem cell-related gene CD34 was conducted in intermuscular ADMSCs cultured until passage 5 using real-time PCR. Regardless of which tissue dissociation protocol was used, increased transcription of CD44 and CD73 genes and decreased transcription of the CD34 gene were observed in intermuscular ADMSCs cultured until passage 5. All data shown represent mean ± SEM of three independent experiments.