Table 1

Summary of primer sequences for RT-PCR and real-time RT-PCR and expected product sizes

Primer	Sequence of forward (F) and reverse (R) primers (5’ → 3’)	Annealing temperature (℃)	Product size (bp)	GenBank accession no.
CD127	F: TCCGCTGTCCCGATTTCTAC	62	289	NM_001146128.2
	R: TTACTTTCCCTGCTGCCTCC
BCL11B	F: TCAAGAACTGCAGCAACCTG	60	255	NM_001297633.1
	R: GCTCGATTTTGACGTCGTTAG
RORC	F: GAAGTGGTGCTGGTCAGGAT	60	140	XM_013997125.2
	R: CGGGAGAAGTCAAAGATGGA
RPL7	F: AAGCCAAGCACTATCACAAGGAATACA	60	172	NM_001113217
	R: TGC ACACCTTTCTGACCTTTG
UBB	F: GCATTGTTGGCGGTTTCG	60	81	NM_001105309.1
	R: AGACGCTGTGAAGCCAATCA
TBP	F: AACAGTTCAGTAGTTATGAGCCAGA	60	262	DQ845178.1
	R: AGATGTTCTCAAACGCTTCG

Journal of Animal Reproduction and Biotechnology 2023;38:89-98 https://doi.org/10.12750/JARB.38.3.89