Expression of CD127, BCL11B and RORC in conceptuses from Days 12 and 15 of pregnancy and in chorioallantoic tissues during mid- to late pregnancy. (A) RT-PCR analysis of ILC markers in conceptuses on Days 12 and 15 of pregnancy. RPL7 was used as a positive control for PCR reaction. RTase +/-, with (+) or without (-) reverse transcriptase; M, molecular marker; D12 Endo, endometrium on Day 12 of pregnancy; D12 Con, Day 12 conceptus; D15 Con, Day 15 conceptus. (B) Real-time RT-PCR analysis of the expression of ILC markers in chorioallantoic tissues on Days 30, 60, 90, and 114 of pregnancy. Data are reported as expression relative to that detected on Day 30 of pregnancy after normalization of the transcript amount to the endogenous ribosomal protein L7 (RPL7), ubiquitin B (UBB), and TATA binding protein (TBP) controls, and data are presented as means with standard errors.