Effects of IFNG on CD127 (A), BCL11B (B), and RORC (C) in endometrial explant cultures. Endometrial explants from gilts on Day 12 of the estrous cycle were cultured with increasing doses of IFNG (0, 1, 10, and 100 ng/mL) in the presence of E2 (10 ng/mL), P4 (30 ng/mL), and IL1B (10 ng/mL). Abundance of mRNA expression based on real-time RT-PCR analyses is relative to that for ILC markers mRNA in the control group of endometrial explants after normalization of transcript amounts to ribosomal protein L7 (RPL7), ubiquitin B (UBB), and TATA binding protein (TBP) mRNAs. Data are presented as means with standard errors. These treatments were performed in triplicate using tissues obtained from each of three gilts. ns, not significant.
pISSN: 2671-4639
