Morphology of porcine induced pluripotent stem cells (piPSCs) cultured in differentiation media. (A) Morphologies of porcine induced pluripotent stem cells (piPSCs: OSKMNL) cultured in stem cell medium and four differentiation media [StemDiff APELTM 2 medium (APEL), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) on days 2, 5 and 8. (B) AP activity of piPSCs (OSKMNL) cultured on iMEF at passage 3 and 20. Scale bar = 200 µm.
pISSN: 2671-4639
