Expression of pluripotency-associated genes and endothelial cell marker in differentied cells derived from piPSCs cultured in diffrentiation media. Relative mRNA level of pluripotency-associated genes (OCT-3/4, SOX2 and NANOG) and endothelial cell marker (CD-31) in porcine differentied cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APELTM 2 medium (APEL), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Values presented as mean SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus differentiaion media. Control: piPSCs (OSKMNL).
pISSN: 2671-4639
