Immunocytochemistry of pluripotency-associated markers in differentiated cells derived from piPSCs cultured in differentiation media. Immunostaining images of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APELTM 2 medium (APEL), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Blue: Staining of Hoechst 33342, Red: Staining of OCT-3/4, SOX2 and NANOG protein. Merge: Hoechst 33342 signal and OCT-3/4, SOX2 and NANOG protein. Scale bar = 50 µm.
pISSN: 2671-4639
