Expression of endothelial cell marker (CD-31) protein in differentiated cells derived from piPSCs cultured in differentiation media. (A) Immunocytochemistry of endothelial cell marker (CD-31) protein in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APELTM 2 medium (APEL), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Blue: Hoechst 33342 signal for nuclei. Red: Staining of vascular endothelial cells marker (CD-31) protein. Merge: Hoechst 33342 signal and CD-31 protein. Scale bar = 50 µm. (B) Western blotting of endothelial cell marker (CD-31) protein in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media (AEPL-2, APEL-2 + VEGF, EBM-2 and EBM-2 + VEGF) for 8 days.
pISSN: 2671-4639
