Flow cytometry of endothelial cell marker (CD-31) in differentiated cells derived from piPSCs cultured in differentiation media. Flow cytometry of endothelial cell marker (CD-31) expression in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APELTM 2 medium (APEL), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) and Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days was analyzed. Swine umbilical cord vein endothelial cells (SUVEC) were used as the positive control. Cells in blue were treated with PE-conjugated CD-31 and cells in red were not treated with PE-conjugated CD-31.
pISSN: 2671-4639
