Comparison of adhesion and proliferation ability of ES cells according to gelatin coating concentration. (A) Mouse ES cells (E14, 5 × 104 cells) were seeded and incubated on petri-dishes coated with various concentrations of gelatin for 48 h, then the morphology of each ES colony was captured. Black arrow indicates clumped cell mass and white arrow indicates attached colony. (B) Mouse ES cells (E14, 5 × 103 cells) were seeded on each concentration of gelatin-coated 96-well culture plate. Purchased cell culture dishes are coated with basic poly-L lysine. After 48 h, cell proliferation analysis was performed using CCK reagent. Data were normalized to control and presented as mean ± SEM of N = 5 experiments. The CCK analysis was independently repeated three times. **p < 0.01 vs. control (no coating).