Comparison of ES cell differentiation at each gelatin coating concentration. (A) Mouse ESCs (E14, 5 × 104 cells) were seeded and incubated gelatin-coated (various concentrations) 12-well culture plate. Purchased cell culture dishes were coated with basic poly-L lysine. For differentiation induction, ES cells were treated with a differentiation induction medium supplemented with 1 μM retinoic acid for 4 days. The black arrow indicates abnormal morphology in the ES colony and the white arrow indicates a normal morphology of differentiated cells. (B) OG2 ESCs (5 × 104 cells) were seeded and incubated gelatin-coated (various concentrations) 12-well culture plate. For differentiation, OG2 were treated with a differentiation induction medium supplemented with 1 μM retinoic acid for 4 days. 4 days after differentiation induction, OG2 was observed using a fluorescence microscope.
pISSN: 2671-4639
