Journal of Animal Reproduction and Biotechnology

Table. 3.

Table 3

Developmental ability by supplement of FBS or SCM in CR1aa medium after microinjection of medium into cytoplasm of in vitro fertilized zygotes

Treatment*		No. of zygotes fertilized	No. (%) of embryos cleaved**	No. (%) of blastocysts/cleaved embryos	No. (%) of hatching blastocysts/cleaved embryos
Control	BSA-FBS	256	220 (85.9 ± 2.0)^a	80 (36.3 ± 2.1)^a	45 (20.3 ± 2.0)^a
Cytoplasm injection	BSA-FBS	512	392 (76.4 ± 2.1)^b	54/196 (27.6 ± 2.1)^b	26 (13.3 ± 2.3)^b
Cytoplasm injection	BSA-SCM	512	392 (76.4 ± 2.1)^b	67/196 (34.2 ± 2.9)^a	37 (18.9 ± 2.7)^a

*Cleaved embryos cultured in CR1aa medium supplemented with 0.3% fatty acid BSA during day 3 after in vitro fertilization were cultured in each CR1aa medium supplemented with 10% FBS or SCM.

**Each half of cytoplasmic microinjected cleaved embryos of medium were cultured in each CR1aa medium supplemented with FBS or SCM.

Values with different superscripts (^{a, b}) within column differ significantly (p < 0.05).

Journal of Animal Reproduction and Biotechnology 2023;38:143-50 https://doi.org/10.12750/JARB.38.3.143