Journal of Animal Reproduction and Biotechnology

Table. 4.

Table 4

Developmental ability by supplement of FBS or SCM in CR1aa medium after microinjection of medium into pronucleus of in vitro fertilized zygotes

Treatment*		No. of zygotes fertilized	No. (%) of embryos cleaved**	No. (%) of blastocysts/cleaved embryos	No. (%) of hatching blastocysts/cleaved embryos
Control	BSA-FBS	198	169 (84.8 ± 2.4)^a	60 (35.5 ± 3.7)^a	34 (19.9 ± 3.3)^a
Pronucleus injection	BSA-FBS	398	282 (70.8 ± 2.4)^b	38/141 (26.9 ± 2.5)^b	16 (11.4 ± 2.0)^b
Pronucleus injection	BSA-SCM	398	282 (70.8 ± 2.4)^b	47/141 (33.4 ± 2.7)^a	25 (17.8 ± 3.3)^a

*Cleaved embryos cultured in CR1aa medium supplemented with 0.3% fatty acid BSA during day 3 after in vitro fertilization were cultured in each CR1aa medium supplemented with 10% FBS or SCM.

**Each half of pronucleus microinjected cleaved embryos of medium were cultured in each CR1aa medium supplemented with FBS or SCM.

Values with different superscripts (^{a, b}) within column differ significantly (p < 0.05).

Journal of Animal Reproduction and Biotechnology 2023;38:143-50 https://doi.org/10.12750/JARB.38.3.143