Isolation of Hs-GEBV and Ls-GEBV bovine satellite cells and comparative analysis of characteristics of Hs-GEBV and Ls-GEBV bovine satellite cells in vitro at short-term culture. (A) Isolation of Hs-GEBV and Ls-GEBV bovine satellite cells by using bovine satellite cells specific marker CD29 and CD56. The CD29 and CD56 positive cells were sorted by FACS sorting. (B) Morphology and immunocytochemistry of Hs-GEBV and Ls-GEBV satellite cells at short-term culture were stained with Pax7 (green) antibody and DAPI (blue). Scale bar 100 μm. (C) The proliferation of Hs-GEBV and Ls-GEBV bovine satellite cells at short-term culture. The analysis was performed after treating with CCK-8 for 3 hours. n = 12, The asterisks represent significant differences in expression levels (Student’s t-test): *p < 0.05; **p < 0.01; ***p < 0.001. (D) Gene expression of Pax7 and MyoD1 in Hs-GEBV and Ls-GEBV bovine satellite cells at short-term culture. n = 3, The asterisks represent significant differences in expression levels (Student’s t-test): ***p < 0.001.
pISSN: 2671-4639
