Comparative analysis of characteristics of Hs-GEBV and Ls-GEBV bovine satellite cells in vitro at long-term culture. (A) Morphology and immunocytochemistry of Hs-GEBV and Ls-GEBV satellite cells at long-term culture with Pax7 (green) antibody and DAPI (blue). Scale bar 100 μm. (B) The proliferation of Hs-GEBV and Ls-GEBV bovine satellite cells at long-term culture. The analysis was performed after treating with CCK-8 for 3 hours. n = 12, The asterisks represent significant differences in expression levels (Student’s t-test): *p < 0.05. (C) Gene expression of Pax7 and MyoD1 in Hs-GEBV and Ls-GEBV bovine satellite cells at long-term culture. (D) Gene expression of Pax3 in Hs-GEBV and Ls-GEBV bovine satellite cells at short- and long-term culture. n = 3, Each letter (a, b, c, d) represents significant differences (p < 0.0001).
pISSN: 2671-4639
