Growth performance on 3D cultivation of porcine jejunum-derived intestinal organoids. (A) Porcine jejunum-derived intestinal organoids substantially grown in 3D showing spheroidal (round-shaped) morphology on day 2 and mature villi and crypt-like structures on day 5 at each passage. Spherical: 50 µm, Branched: 50 µm. (B) Porcine jejunum-derived intestinal organoids showed consistent growth on an average of 140-150 organoids per basement matrix dome from P1 to P10 at each generation, indicating continuous proliferation and long-term maintenance and showing high differentiation capacity for maturation on day 14. Scale bar: 200 µm.
pISSN: 2671-4639
