Characterisation and paracellular permeability of porcine jejunum-derived intestinal organoids. (A) Immunostaining of LGR5, Cytokeratin 19, and Mucin2 in porcine jejunum-derived intestinal organoids at passage 5. Organoids were counterstained with DAPI. Scale bar: 100 μm. (B) Paracellular permeability of the epithelial layer in porcine jejunum-derived intestinal organoids using fluorescent tracers. Scale bar: 100 µm.