Characterization of ovarian cell aggregates according to cultured ovarian cell populations. Ovarian cell aggregates derived from two ovarian cell populations from top to 50% and 20 to 40% layers after Percoll density gradient centrifugation were subjected to histological and gene expression analyses. (A) Histological observation. Pre-vitellogenic oocytes were observed in ovarian cell aggregates derived from top to 50% layers but not in those from 20 to 40% layers. Intact ovarian tissues were used as a positive control. Scale bar = 100 μm. (B) RT-PCR analysis for several genes expressed in ovary. Expression of genes specific for germline cells (nanos2 and scp3) and somatic cells (fshr, lhr, and foxl2) was evaluated by RT-PCR analysis. Except for lhr gene, all genes were expressed in ovarian cell aggregates regardless of its original cell populations. Expression of lhr gene was detected only in ovarian cell aggregates derived from top to 50% layers.
pISSN: 2671-4639
