Evaluation of the effects of media supplements on the culture of ovarian cell aggregates. Ovarian cell aggregate culture was performed in mESM2 and mESM2 without FS, bFGF, and EE and after ovarian cell aggregate formation, its viability, aggregate size, and relative mRNA level for nanos2 and scp3 were measured. (A) Representative images of ovarian cell aggregates cultured in each media for 7 days. No significant difference was observed in morphology between two groups. Scale bar = 250 μm. (B, C) Viability of ovarian cell aggregates. Live/dead staining (B) and trypan blue assay (C) were performed to visualize and compare the viability of the cells comprising aggregates cultured in each media. No significant difference was observed between two groups. Scale bar = 200 μm. (D) Size of ovarian cell aggregates. No significant difference was observed between two groups. (E) Relative mRNA expression of nanos2 and scp3 in ovarian cell aggregates. Expression of nanos2 did not show significant difference between two groups, but scp3 decreased in the group cultured without FS, bFGF, and EE. All values are expressed as mean ± standard deviation of three independent experiments. Asterisk (*) indicates significant difference (p < 0.05).
pISSN: 2671-4639
