Investigation of the effects of bFGF and GDNF on the expression of nanos2 and scp3 in ovarian cell aggregate culture. Two factors were added alone or together to culture media (mESM2) without FF, bFGF, and EE and after culture for 7 days, mRNA expression levels of both genes in ovarian cell aggregates formed were measured by qRT-PCR. Both bFGF and GDNF increased nanos2 mRNA expression. In case of scp3, the highest expression was observed when bFGF was added alone. All values are expressed as mean ± standard deviation of three independent experiments. abcdeDifferent letters indicates significant differences (p < 0.05).