Journal of Animal Reproduction and Biotechnology

Table. 1.

Table 1

Comparison of frozen–thawed sperm motility for different numbers of sperm in straws

No. spermatozoa/straw (replicates)	10 million (15)	15 million (15)	18 million (15)	20 million (15)
Total motile (%)	78.3 ± 5.3^b	82.2 ± 4.4^a	83.3 ± 5.3^a	85.0 ± 6.8^a
Fast progressive (%)	36.1 ± 5.5^a	35.0 ± 2.9^ab	31.9 ± 5.1^bc	28.3 ± 5.7^c
Slow progressive (%)	20.2 ± 3.9^b	23.0 ± 3.0^ab	23.6 ± 3.7^ab	25.4 ± 8.7^a
Non-progressive (%)	22.0 ± 2.9^c	24.2 ± 2.0^c	27.7 ± 2.7^b	31.3 ± 3.9^a
Immotile (%)	21.7 ± 5.3^a	17.8 ± 4.4^b	16.7 ± 5.3^b	15.0 ± 6.8^b
VCL (μm/s)	90.7 ± 8.7^a	81.8 ± 4.4^b	77.8 ± 6.1^b	77.6 ± 8.9^b
VSL (μm/s)	39.1 ± 3.1^a	34.0 ± 3.2^b	31.7 ± 4.1^bc	30.4 ± 3.8^c
VAP (μm/s)	53.3 ± 4.4^a	47.6 ± 3.6^b	45.4 ± 4.3^b	44.9 ± 3.8^b
LIN (%)	43.4 ± 4.2^a	41.5 ± 2.2^ab	40.7 ± 3.2^ab	39.4 ± 5.6^b
STR (%)	73.5 ± 4.0^a	71.3 ± 2.1^ab	69.6 ± 3.3^bc	67.6 ± 5.7^c
WOB (%)	58.9 ± 2.8	58.2 ± 1.7	58.4 ± 2.2	58.1 ± 3.5
ALH (μm)	3.3 ± 0.4	3.2 ± 0.2	3.1 ± 0.2	3.1 ± 0.4
BCF (Hz)	17.0 ± 1.1^a	15.4 ± 1.4^b	15.3 ± 1.4^b	15.4 ± 1.6^b
Hyperactive (%)	16.0 ± 3.1^a	14.7 ± 1.9^a	12.0 ± 2.8^b	10.4 ± 3.2^b

Values are presented as the means ± standard deviation (SD). ^a,bValues (mean ± SD) in rows denoted by different superscript lower-case letters differ significantly among groups (p < 0.05). In total, 15 freeze-thawed straws derived from five bulls (F, G, H, I, and J) were used to evaluate sperm motility and motility parameters. Three straws from each bull were used in the experiment. Total motile (%), Progressive motile (%), Slow progressive (%), Non-progressive (%), Immotile (%), curvilinear velocity (VCL, μm/s), straight-line velocity (VSL, μm/s), average path velocity (VAP, μm/s), linearity (LIN = VSL/VCL, %), straightness (STR = VSL/VAP, %), amplitude of lateral head (ALH, μm/s), and flagellar beat cross frequency (BCF, Hz), and hyperactivity (%).

Journal of Animal Reproduction and Biotechnology 2024;39:40-7 https://doi.org/10.12750/JARB.39.1.40