pISSN: 2671-4639
eISSN: 2671-4663
pISSN: 2671-4639
eISSN: 2671-4663
The viability and acrosomal membrane integrity of frozen–thawed spermatozoa at different concentrations in straws
| No. sperm/straw | Replicate | LIA | LDA | DIA | DDA |
|---|---|---|---|---|---|
| 10 million | 15 | 63.8 ± 10.7 | 0 ± 0 | 34.1 ± 10.4 | 2.1 ± 1.3 |
| 15 million | 15 | 67.7 ± 5.5 | 0.1 ± 0.2 | 29.5 ± 4.8 | 2.7 ± 1.9 |
| 18 million | 15 | 71.7 ± 6.8 | 0.1 ± 0.2 | 26.8 ± 6.4 | 1.5 ± 0.9 |
| 20 million | 15 | 70.1 ± 8.6 | 0.1 ± 0.3 | 28.3 ± 8.4 | 1.6 ± 1.1 |
Frozen-thawed semen was collected from five bulls (F, G, H, I, and J), and three straws per bull were used to evaluate viability and acrosomal membrane integrity. LIA, live spermatozoa with intact acrosomes; LDA, live spermatozoa with damaged acrosomes; DIA, dead spermatozoa with intact acrosomes; DDA, dead spermatozoa with damaged acrosomes.