Effect of cadmium exposure on mitochondrial function in porcine embryos. (A) Bright-field images and MitoTracker fluorescence images of day 2 embryos. Bar = 200 µm. (B) Quantification of MitoTracker fluorescence intensity (n = 50 per group). (C) Bright-field images and TMRM fluorescence images of day 2 embryos. Bar = 200 µm. (D) Quantification of TMRM fluorescence intensity (n = 52 per group). (E) Bright-field and MitoSOX fluorescence images of day 2 embryos. Bar = 200 µm. (F) Quantification of MitoSOX fluorescence intensity (n = 52 per group). (G) Relative expression of mitochondrial genes in day 2 embryos (n = 3 per group). Values with different superscript letters (a and b) differ significantly (p < 0.05). Data are compiled from three independent experiments.