Analysis of differentially expressed genes (DEGs) in POA porcine oocytes. (A) Schematic diagram of experimental design. All the porcine oocytes were cultured for 44 of IVM (Control) or 68 h of IVM (POA or in vitro aging groups; with 24 h additional culture after 44 h of IVM). (B) DEGs or the number of genes showing differential expression between IVM and POA porcine oocytes. Functional annotation analysis of DEGs in porcine oocytes from POA group. Biological processes (red), cellular components (green), and molecular functions (blue) are indicated. (C-F) Gene ontology (GO) enrichment analysis of the DEGs associated with the expression of “Oxidative stress,” “Mitochondrial functions and fission,” “Mitophagy and autophagy,” and “Apoptotic process,”-related genes in POA-induced model. (G-J) POA compared with IVM porcine oocytes. Each heatmap cluster illustrates the expression of various genes differentially expressed (upregulated: red; downregulated: blue) between the IVM and POA-induced model. Heatmap clusters are analyzed to derive the “Oxidative stress,” “Mitochondrial functions and fission,” “Mitophagy and autophagy,” and “Apoptotic process” categories.
pISSN: 2671-4639
