Changes in DRP1-mediated mitochondrial fission on POA porcine oocytes. Changes of mitochondrial dynamics in porcine POA oocytes compared with IVM oocytes. (A, B) Stained porcine oocytes of IVM (44 h of IVM) and POA group (68 h of IVM) exhibited mitochondrial fragmentation (< 1 μm) and elongation (> 3 μm) using MitoTracker green staining. Scale bar: 50 μm. (C, D) Investigation of mitochondria fluorescence intensity from POA porcine oocytes using MitoTracker green staining. Scale bar: 50 μm. (E-H) Representative Western blot and quantitative analyses of the protein levels of mitochondrial fission regulator [pDRP1-Ser616 and DRP1] in IVM porcine oocyte and POA aged oocytes. Western blot analysis of cytochrome C protein expression as a mitochondrial-mediated apoptotic factor in POA model compared to IVM oocytes. The relative expression of all proteins is normalized to that of β-actin. Image data and Western blot data were respectively expressed as mean ± SD/SEM and were analyzed using a t-test. Differences are considered significant at *p < 0.05, **p < 0.01 and ***p < 0.001.
pISSN: 2671-4639
