Analysis of testicular tissue sections of PDGFRαEGFP mice. (A) Hematoxylin and Eosin (H&E) staining depicts typical seminiferous tubule architecture with visible spermatogenic, Sertoli and Leydig cells. (B) Fluorescent imaging of testicular tissue of PDGFRαEGFP mice. DAPI staining highlights nuclei within the testicular tissue. (C) Magnified fluorescent imaging shows PDGFRα-positive cells (green) in the interstitial space with specific localization in peritubular myoid cells (PMCs, red arrow) and Leydig cells (yellow arrow). (D) From left to right, DAPI nuclear staining, α-SMA cytoskeletal staining in PMCs, PDGFRα staining in interstitial cells, combined staining of α-SMA/PDGFRα/DAPI, and Differential Interference Contrast (DIC) microscopy for tissue structure visualization. The co-localization of α-SMA and PDGFRα (indicated by the overlay of green and red resulting in yellow) suggests the presence of both markers in the same cells. Scale bars, 100 μm.
pISSN: 2671-4639
