pISSN: 2671-4639
eISSN: 2671-4663
pISSN: 2671-4639
eISSN: 2671-4663
The effect of different thawing temperatures and durations on motility kinematic parameters of dog sperm frozen in cryovials
| Temperature | Time | VCL (µm/s) | VAP (µm/s) | VSL (µm/s) | STR (%) | LIN (%) | WOB (%) | ALH (µm/s) | BCF (Hz) |
|---|---|---|---|---|---|---|---|---|---|
| 37℃ | 60 sec | 34.2 ± 1.5 | 26.2 ± 1.9 | 21.9 ± 2.0 | 75.5 ± 2.0 | 56.6 ± 1.9 | 74.8 ± 1.3 | 1.3 ± 0.0 | 6.4 ± 0.2 |
| 37℃ | 60 sec. Stir | 40.5 ± 2.5 | 34.2 ± 2.3 | 30.7 ± 2.5 | 76.6 ± 1.6 | 64.0 ± 1.4 | 69.9 ± 10.2 | 1.2 ± 0.0 | 7.0 ± 0.3 |
| 24℃ | 24 min | 34.3 ± 2.0 | 28.2 ± 1.7 | 24.5 ± 1.9 | 76.7 ± 0.6 | 62.4 ± 1.5 | 78.2 ± 1.3 | 1.2 ± 0.0 | 6.2 ± 0.2 |
| 75℃ | 20 sec | 35.2 ± 0.4 | 29.0 ± 0.1 | 24.9 ± 0.4 | 73.8 ± 3.3 | 60.0 ± 3.4 | 77.3 ± 2.0 | 1.2 ± 0.0 | 6.1 ± 0.0 |
Stir, stirring; VCL, curvilinear velocity; VAP, average path velocity; VSL, straight-line velocity; STR, straightness; LIN, linearity; WOB, wobble; ALH, amplitude of the lateral head displacement; BCF, beat cross frequency. Values are expressed as the mean ± SE.