Effects of different concentrations of AFB1 on CSIc viability after treatment for 24 h. (A) Cell viability and (B) cytotoxicity assay of CSIc treated with AFB1 for 24 h. Data are presented as the mean ± SEM; Student’s t-test, n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 versus CON. AFB1, aflatoxin B1; CSIc, canine small intestinal epithelial primary cells; CON, control.