Effects of different concentrations of AFB1 on the mRNA expression of tight junction-related genes including (A) CLDN3, (B) OCLN, (C) MUC2, and (D) TJP1 in canine small intestinal epithelial primary cells. Data are presented as the mean ± SEM (n = 4). Different letters (ab, bc, and c) above the bars indicate significant differences between means; ab and ac indicate insignificant difference. Additionally, if the ANOVA was not statistically significant, no letter is displayed on the graph. AFB1, aflatoxin B1; CON, control; CLDN3, claudin 3; OCLN, occludin; MUC2, mucin 2; TJP1, tight junction protein 1.
pISSN: 2671-4639
