Effect of catechin hydrate on cisplatin-induced GC-1 spg cell damage. (A) The chemical structure of catechin hydrate. (B) Cell viability was measured in GC-1 spg cells following treatment with 0-100 μM catechin hydrate, or 10 μM cisplatin with 0-100 μM catechin hydrate, and cultured for 24 h. The graph shows the mean ± SEM of three independent experiments. (C) Immunostaining of Ki67 in GC-1 spg cells. (D) Relative percentage of Ki-67-positive cells shown as a graph with the mean ± SEM of three different experiments. *p < 0.05; **p < 0.01, compared to controls. Scale bar = 50 μm.