pISSN: 2671-4639
eISSN: 2671-4663
The sequence of primers used in qRT-PCR
Target gene | Primer sequence | Product size (bp) | Annealing temp (℃) | Accession number |
---|---|---|---|---|
Bax | F:TTTGCTTCAGGGTTTCATCC | 146 | 60 | NM_001003011.1 |
R:TGTTACTGTCCAGTTCATCTCC | ||||
Bak | F:TCTACTTCTGAGGAGCAGGTAGC | 153 | 60 | NM_001020808.1 |
R:CATGGTGCTGCTAGGTTCTAGG | ||||
Bcl2 | F:GGGTCATGTGTGTGGAGAGC | 180 | 60 | NM_001002949.1 |
R:GCCAGGAGAAGTCAAACAGAGG | ||||
Birc5 | F:ACATTCATCTGGTTGTGCTTTCC | 157 | 60 | NM_001003348.1 |
R:CACTTTCTTTGCGGTCTCTTCG | ||||
Hsp70 | F:GGTGCAGGTGAGCTACAAGG | 158 | 60 | NM_001003067.2 |
R:GCTGCGAGTCGTTGAAGTAGG | ||||
Hsp90 | F:CGTGGAAAGAATGAAGGAGAAGC | 146 | 60 | NM_001003327.2 |
R:AGTATTCGTCCACAGGTTCGG | ||||
Oct4 | F:AACGATCAAGCAGTGACTATTCG | 147 | 60 | XM538830.1 |
R:AGTAGAGCGTAGTGAAGTGAGG | ||||
Sox2 | F:AGTCTCCAAGCGACGAAAAA | 189 | 60 | DR105272 |
R:CCACGTTTGCAACTGTCCTA | ||||
Nanog | F:GACCGTCTCTCCTCTTCCTTCC | 157 | 60 | XM_014108418.1 |
R:CGTCCTCATCTTCTGTTTCTTGC | ||||
Hprt1 | F:TCATCATTACGCTGAGGATTTGG | 150 | 60 | NM_001003357.2 |
R:AAGAATTTATAGCCTCCCTTGAGC |
Bax, BCL2 associated X; Bak, BCL2 antagonist/killer 1; BCL2, B-cell leukemia/lymphoma 2; Birc5, baculoviral IAP repeat-containing 5; Hsp70, heat shock protein 70; Hsp90, heat shock protein 90; Oct4, POU class 5 homeobox 1; Sox2, SRY-box transcription factor 2; Nanog, Nanog homeobox; Hprt1, hypoxanthine phosphoribosyltransferase 1.