Activation and localization of MMPs and analysis of expression patterns of MMP-related proteins in embryos of apple seed extract-treated or untreated groups. (A) Gelatin zymorgraphy, (B) enzyme-linked immunosorbent assay (ELISA) results of MMP activation and inhibitor factor protein. NC, non-coculture medium culture system; NA, non-coculture medium (with ASE) culture system with apple seed extract; CC, coculture system; CA, coculture system with apple seed extract. The symbol * indicates significant difference at the 0.05 level (p < 0.05).